You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. U-bottom: 30-40 µl. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. 384- or 96-well read plate containing cells loaded. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. 100 fmol 384-well (75 µL) 0. Integrated stacker option. 4 decades . Molecular Devices Spectramax. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Algorithms are optimized for accurate cell detection and. VIS absorbance detection. The. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. 1. Plate formats. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Artel MVS Bundle DM Std/800TSNB Reader Details. Biotek Synergy 2 Plate Reader Multi-Mode. EPOCH2 Plate Reader/Spectrophotometer. Application Area: Spectrophotometer for molecular biology. 96-, 384-, and 1536. Artel MVS Bundle DM Std/800TSNB Reader Details. Page 1 of 8 . With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. #6590-0118) Filter 665/10 (Molecular Devices cat. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. com Check our website for a current listing of worldwide distributors. +1 800. This unlocks the transport slide. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 340 PC384. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. x. X. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. 9. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. A thermal cycling reaction was immediately initiated. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 2, prime the machine with CellTiter-Glo ® 2. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. RUO. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. x. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. 0. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. A rinse program is included for quick and easy daily. * Note: Programming the washer with a final aspirate height of 4. A = 2 - log 10 (%T). " A versatile UV/VIS spectrophotometer for both 96- and 384-well. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Streamline your workflow with comparative confluence assays to identify and verify gene edits. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. QC tools and IQOQ services. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 2 OD at 200 nm; this decreased to 0. MultiWash+ Microplate Washer. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. x. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. #6590-0118) Filter 665/10 (Molecular Devices cat. X. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. In addition, SoftMax Pro SoftwareAfter incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. 96-well fluorescent cell dilution series, top-read. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. $913. 5 ng per well. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. SpectraMax ID5 Plate Reader (Molecular Devices). Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 0. Uncontrolled Copy for Training and Reference Purposes Only . Cells toxicity was corrected for the culture medium background absorbance. This can increase throughput and efficiency, thus saving time and space in the lab. flat meniscus), but is approx 15 mL. 20 attomol ATP per well. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. 2. #3-000-510)Corning® 384 well microplate, low volume poly-D-lysine coated surface, black polystyrene, round bottom, sterile, lid, pack of 10; Synonyms: 384 multiwell plates,384 well low volume plates,384 well microplates,384 well microtiter plates,384 well plates,384 well tissue culture treated plates; find -CLS3643 MSDS, related peer-reviewed papers,. Published Dec 16, 2020 | Updated Dec 16, 2020. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Select Start>Control panel>Device Manager. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. x. $245. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 96-, 384-, and 1536-well microplates. The opaque walls prevent stray light from being detected from neighbouring. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. 00. 100 fmol luciferase per well. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. We also prefer that fluorescence signals in one well don’t bleed into the other, as this means less background and more precise data. 2. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. x. 96- and 384-well microplates. 2. With optimized reagents, option to operate the readers. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer. We would like to show you a description here but the site won’t allow us. Assay setup for a 384-well low-volume plate. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. VMax . The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 96-384. M5. x. These models can no longer be repaired. Analyst, M5, Flex 3, and SpectraMax L readers work differently. A snapshot of the Settings screen is shown in Figure 2. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 3. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. It can be performed using any well in the plate with a relatively strong fluorescent signal (i. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. Following the flushing of the machine as described in Section 3. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Page 154: Ordering Parts And Accessories. MultiWash+ Microplate Washer. Find our technical support information along with contact details. using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Next generation SpectraMax® absorbance microplate readers. 25mm: Plate height:Absorbance was measured at 490 nm with SpectraMax Plus 384 plate reader (Molecular Devices, San Jose, CA). #4TI-0655) Microplates. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. M5. x. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. Features. 616, 665 nm EM filters 0200-7011. 1 pM 1536-well: 0. Streamline GLP/GMP compliance. Temperature control: 4°C above ambient to 45°C. Reliable performance with a proven track record. 384-well TC plate, Poly-d-Lysine, Flat. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. • Tyrosine Kinase Plate Please contact your Molecular Devices sales representative. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. To update the driver. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 00. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. No accessories are needed and SoftMax® Pro Software. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Microplates, cuvettes (via adapter) Detectors. They perform rapid throughput cell-based assays while. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Shaking: Linear, orbital, and double orbital. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Visit the supplier page or submit a quote to learn more. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Round well design with a clear flat bottom 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. 48 W x 12. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax iD5. 4 Equipment. Experience the identifiably different. For more sample throughput, the system canabsorbance plate readers. M2. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. ©2022. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 100 fmol 1536-well (8 µL) 0. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 96-, 384-, and 1536-well microplates. #ABS Plus). Select Yes at the warning. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. [Carbachol] (nM) RFU Max. x. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. These robust readers have been placed in labs from Antarctica to the. 2. Selects either 96-well or 384-well plate for a stand-alone read. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 1 fmol luciferase per well. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. The drawing shows a standard microplate of 15 mm height covered with a lid. Our microplate. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. ,LTD (CAT#: STEM-S. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. the iD5 reader’s filter system. x. * Note: Programming the washer with a final aspirate height of 4. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). Suggested plates are black. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. 7°C (gradient heating design). Fluid dispensing speed. x. 96/384-well plate formats. Plus 384. The plate was also read at z height 1 mm with varying delay and integration times for the donor and acceptor reads. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. The VICTOR Nivo. A ten-fold serial dilution of purified luciferase in PBS with 0. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 305 reference level saturation. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. Plus 384. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. UV absorbance detection. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Instrument pricing. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. Loading Buffer was also added to a set of cell-free wells for background controls. Microplates, cuvettes (via adapter) Detectors. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. Use the provided 3. Contact our support team with your questions regarding Molecular Devices products and applications. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. e. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. begin reading microplates with seven mouse clicks. both 96- and 384-well plates and comes with four wash/rinse bottles and one waste. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. –Min. #W50-100). #0200-7011) Instrument setupRound well design with a solid black flat bottom2. Molecular Devices: n/a:. ,LTD (CAT#: STEM-S. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. This enhanced capability. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Visible absorbance -. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes<2 fg/well for firefly luciferase in 96- and 384- well top read. 2 with 1000 cells. x. Ensure data accuracy of your reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. measurements in compatible Molecular Devices® microplate readers. Round well design with a solid black flat bottom2. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Filter. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 00. SpectraMax® Plus 384 provides ultrafast, full spectral range. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Select Update Driver. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. There is no product in the shopping cart, buy it!. Service Contracts, Preventative Maintenance and. ) 0. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. flat-bottom: 50-60 µl. Outer wells aligned for proper viewing with FLIPR Ideal. Dimensions: 18. 00. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. The plate reader optics are tunable for. 003 fmol 0. XML file. Read one sample or up to 384. Select Consumables. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Automated live cell imaging – cell counting and confluence. com. The SpectraTest validation. The system provides automation for up to 50 microplates for easy walkaway automation. 5577. 4 Equipment. Others models hold the sample in place using surface tension. ©2013 Molecular Devices, LLC • 6/13 • Printed in USA • PN: 0120-1523. Molecular Devices makes. After the reaction was complete, 5 µL of each sample was transferred into wells of a 384-well plate and detected on the microplate readers using the instrument settings listed in Table 1. ability to use as little as 2˜µL samples with 24- or 64-well plates. Fluorescence Microplate Reader Comparison Chart. System setup and calibration are controlled from within SoftMax Pro Software. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Dual injectors for kinetic assays. x.